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Synthesis of cRNA probe for in situ hybridization

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  3. Synthesis of cRNA probe for in situ hybridization

Currently, a sequence region suitable for in situ hybridization probe is quested in the open-reading frame or 3’UTR, and determined appropriately. However, transcriptome analysis based on RNA sequencing has revealed that various levels of exon expression are generated in a given gene. Therefore, in order to obtain a significant result in in situ hybridization, the determination of the probe region is of utmost importance. We propose the candidate regions for probe(underlined with green), based on the customer’s thought.

Exon expression profiles of various transcripts in a given gene
cRNA probe fig.1
Data to verify in silico analysis result by wet experiment
cRNA probe fig.2